The ongoing discovery and characterization of Lactic Acid Bacteria (LAB) for probiotic remains of utmost significance in advancing the field of functional foods. A current literature gap lies in the insufficient exploration of LAB present in plants that are unique in terms of probiotic application. This study evaluated the (1) biochemical, (2) bioactivity, and (3) genomic features of LAB from nipa sap obtained from Pangasinan. Employed research methods includes isolation, gram stain test, catalase test, stress tolerance tests, antagonistic activity, carbohydrate hydrolysis, safety tests consist of hemolysin test and antibiotic susceptibility test, promoter analysis of genes, 16s ribosomal RNA identification, and whole genome sequence (WGS) & genome assembly. The isolate was characterized for gram positive, catalase negative, has γ hemolytic activity, exhibited antagonistic activity to several bacterial pathogens, and resistance to aminoglycosides and lincosamides antibiotics after 24h of incubation. Moreover, 16s ribosomal RNA gene sequencing revealed that the isolate shared an average nucleotide identity of 100% to Lentilactobacillus hilgardii LH500 (NZ_CP044119), the closest related strain identified to date, and have undergone whole genome sequence. Multiple genes that code for stress tolerance, antagonistic activity, and amylase, lipases, & proteases acting as catalysts for the breakdown of macronutrients were detected in the complete genome of L. hilgardiiLH500as confirmatory on its in-vitro activities. Altogether, our findings suggest the potential of nipa sap as a source of probiotic that can be integrated to food products for human consumption. This serves as initial understanding of plant-derived LAB and their biochemical, bioactivity, and genotypic characteristics that can be useful in eradicating infections related to gram negative pathogens, enhancing nutrient digestion, and over-all health.